DNMBP 抗体 (AA 1-115)
Quick Overview for DNMBP 抗体 (AA 1-115) (ABIN7127031)
抗原
See all DNMBP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-115
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特异性
- Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. This MAb recognizes an epitope of alpha-tubulin. The alpha-tubulin is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (aa 1-115) of human Tubulin Alpha protein (exact sequence is proprietary)
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亚型
- IgG1
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Human prostate, lung or colon tissues (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- DNMBP (Dynamin Binding Protein (DNMBP))
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别名
- TUBA
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背景
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Alpha-tubulin 1, Hum a tub1, Hum a tub2, Testis-specific alpha-tubulin, TUBA1, TUBA1A, Tuba4a, Tubulin alpha-1 chain, Tubulin H2-alpha, Tubulin, alpha 1 (testis specific), Tubulin, alpha 4a,Tubulin-alpha (Microtubule Marker)
Cellular localisation: Cell Surface Cytoplasmic -
分子量
- 55kDa
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基因ID
- 7277, 75318
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UniProt
- P68363, P68366
抗原
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