MED22 抗体
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北京 101111
Quick Overview for MED22 抗体 (ABIN7126988)
抗原
See all MED22 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human MED22 protein
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亚型
- IgG2
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anti-Mediator Complex Subunit 22 (MED22) (AA 2-140) antibody
MED22 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 22 (MED22) antibodyMED22 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 22 (MED22) (AA 1-140) antibodyMED22 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Mediator Complex Subunit 22 (MED22) antibodyMED22 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 22 (MED22) (AA 1-100) antibodyMED22 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 3A9 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunofluorescence (0.5-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (0.5-2 μg/million cells), ,Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or U87 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MED22 (Mediator Complex Subunit 22 (MED22))
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别名
- MED22
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背景
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Med24, SURF5, mediator complex subunit 22, Mediator of RNA polymerase II transcription subunit 22,MED22 (Transcription Factor)
Cellular localisation: Nucleus. -
分子量
- 22kDa
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基因ID
- 6837, 78354
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UniProt
- Q15528
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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