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SP100 抗体

SP100 适用: 人 WB, ELISA, IP, IF, Coat, FACS, IHC (f) 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN7126965
发货至: 中国
  • 抗原 See all SP100 抗体
    SP100 (SP100 Nuclear Antigen (SP100))
    适用
    • 37
    • 4
    • 1
    宿主
    • 34
    • 6
    小鼠
    克隆类型
    • 38
    • 2
    单克隆
    标记
    • 23
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SP100 antibody is un-conjugated
    应用范围
    • 32
    • 19
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant full-length human SP100 protein
    亚型
    IgG2b
    Top Product
    Discover our top product SP100 Primary Antibody
  • 应用备注

    Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (0.5-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (0.5-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or U87 cells.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    SP100 (SP100 Nuclear Antigen (SP100))
    别名
    SP100 (SP100 产品)
    别名
    SP100 antibody, A430075G10Rik antibody, lysp100b antibody, SP140 antibody, SP100 nuclear antigen antibody, nuclear antigen Sp100 antibody, nuclear autoantigen Sp-100 antibody, SP100 antibody, Sp100 antibody, LOC101120993 antibody
    背景
    Lysp100b, Nuclear antigen Sp100, Nuclear autoantigen Sp 100, Nuclear dot associated Sp100 protein, SP100, SP100 HMG nuclear autoantigen, SP100 nuclear antigen, Speckled 100 kDa,SP100
    Cellular localisation: Nucleus. PML body.
    分子量
    100.4kDa
    基因ID
    6672, 369056
    UniProt
    P23497
    途径
    Retinoic Acid Receptor Signaling Pathway
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