SP100 抗体
Quick Overview for SP100 抗体 (ABIN7126965)
抗原
See all SP100 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human SP100 protein
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亚型
- IgG2b
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anti-SP100 Nuclear Antigen (SP100) (AA 610-802) antibody
SP100 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 1-98) antibodySP100 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 1-480) antibodySP100 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 1-480) antibodySP100 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 1-480) antibodySP100 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 116-333) antibodySP100 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 1-98) antibodySP100 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) antibodySP100 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 181-480) antibodySP100 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SP100 Nuclear Antigen (SP100) (AA 261-465) antibodySP100 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (0.5-2 μg/mL),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Flow Cytometry (0.5-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or U87 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- SP100 (SP100 Nuclear Antigen (SP100))
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别名
- SP100
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背景
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Lysp100b, Nuclear antigen Sp100, Nuclear autoantigen Sp 100, Nuclear dot associated Sp100 protein, SP100, SP100 HMG nuclear autoantigen, SP100 nuclear antigen, Speckled 100 kDa,SP100
Cellular localisation: Nucleus. PML body. -
分子量
- 100.4kDa
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基因ID
- 6672, 369056
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UniProt
- P23497
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途径
- Retinoic Acid Receptor Signaling Pathway
抗原
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