S100B 抗体
Quick Overview for S100B 抗体 (ABIN7126916)
抗原
See all S100B 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
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交叉反应 （详细）
- Human, Mouse, Rat, Cow.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human S100B protein
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亚型
- IgG2b
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anti-S100 Calcium Binding Protein B (S100B) (AA 31-92) antibody
S100B 适用: 大鼠 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 4C4-9 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4A5H7 unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22934 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow IHC, StM 宿主: 兔 Monoclonal S100B-1706R unconjugated Recombinant Antibody
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human brain or melanoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- S100B (S100 Calcium Binding Protein B (S100B))
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别名
- S100B
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背景
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NEF, Protein S100-B, S-100 protein beta chain, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100beta,S100B (Astrocyte and Melanoma Marker)
Cellular localisation: Cytoplasmic -
分子量
- 10-12kDa
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基因ID
- 6285, 422181
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UniProt
- P04271
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途径
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
抗原
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