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S100A9 抗体

This anti-S100A9 antibody is a 小鼠 单克隆 antibody detecting S100A9 in IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN7126911
发货至: 中国

Quick Overview for S100A9 抗体 (ABIN7126911)

抗原

See all S100A9 抗体
S100A9 (S100 Calcium Binding Protein A9 (S100A9))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This S100A9 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • 特异性

    Recognizes a protein of14 kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant human MRP14 protein

    亚型

    IgM
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Tonsil, lymph node, or spleen

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    S100A9 (S100 Calcium Binding Protein A9 (S100A9))

    别名

    S100A9

    背景

    S100-A9, MRP14, calgranulin-B, calprotectin L1H subunit, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14, S100 calcium-binding protein A9 (calgranulin B),Calprotectin / MRP14 / S100A9 / Calgranulin B
    Cellular localisation: Cytoplasmic

    分子量

    14kDa

    基因ID

    6280

    UniProt

    P06702

    途径

    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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