S100A1 抗体
Quick Overview for S100A1 抗体 (ABIN7126910)
抗原
See all S100A1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Purified human S-100 protein conjugated to methylated BSA
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亚型
- IgG
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应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: T98G, A431 or HT-29 cells. Human brain or melanoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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别名
- S100A1
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背景
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Bpb, NEF, S100-A1, S100 Alpha Chain, S100 Beta Chain, S100 Calcium Binding Protein A1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein Beta Neural,S100A1 (Melanoma Marker)
Cellular localisation: Cytoplasm -
分子量
- 10-12kDa
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基因ID
- 6271, 515715
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UniProt
- P23297
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途径
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
抗原
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