PSMD4/ASF 抗体
Quick Overview for PSMD4/ASF 抗体 (ABIN7126850)
抗原
See all PSMD4/ASF (Psmd4) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human PSMD4protein
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亚型
- IgG2b
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat or HepG2 cells. Liver tissue lysate. Human liver, tonsil or kidney.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))
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别名
- PSMD4
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背景
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26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, AF, AF-1,26S proteasome non-ATPase regulatory subunit 4
Cellular localisation: Nucleus and cytoplasm. -
分子量
- 50kDa
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基因ID
- 5710, 505059
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UniProt
- P55036
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途径
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway
抗原
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