MBNL3 抗体
Quick Overview for MBNL3 抗体 (ABIN7126833)
抗原
See all MBNL3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.
-
交叉反应 （详细）
- Human
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant full-length human MBNL3 protein
-
亚型
- IgG2b
-
-
anti-Muscleblind-Like Splicing Regulator 3 (MBNL3) (AA 116-211) antibody
MBNL3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Muscleblind-Like Splicing Regulator 3 (MBNL3) antibodyMBNL3 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Muscleblind-Like Splicing Regulator 3 (MBNL3) (AA 1-258) antibodyMBNL3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Muscleblind-Like Splicing Regulator 3 (MBNL3) antibodyMBNL3 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or MCF7 cells.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))
-
别名
- MBNL3
-
背景
-
CHCR, Cys3His CCG1 required protein, MBLX, MBLX39, Muscleblind like protein 3, Muscleblind like splicing regulator 3, Muscleblind-like X-linked protein, Protein HCHCR, RP23-98P13.1,MBNL3
Cellular localisation: Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts. -
分子量
- 39kDa
-
基因ID
- 55796
-
UniProt
- Q9NUK0
-
途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
-
