Neurogenin 3 抗体
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北京 101111
Quick Overview for Neurogenin 3 抗体 (ABIN7126754)
抗原
See all Neurogenin 3 (NEUROG3) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1.The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-lengthNEUROG3protein
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亚型
- IgG2b
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anti-Neurogenin 3 (NEUROG3) (AA 40-69), (N-Term) antibody
NEUROG3 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB02913 unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 1-83) antibodyNEUROG3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 1-101) antibodyNEUROG3 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 4E9 unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 1-101) antibodyNEUROG3 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 4B7 unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 61-160) antibodyNEUROG3 适用: 人, 小鼠, 大鼠, Pig WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neurogenin 3 (NEUROG3) antibodyNEUROG3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurogenin 3 (NEUROG3) (N-Term) antibodyNEUROG3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neurogenin 3 (NEUROG3) antibodyNEUROG3 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 93-214) antibodyNEUROG3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurogenin 3 (NEUROG3) (AA 1-214) antibodyNEUROG3 适用: 人 WB 宿主: 小鼠 Monoclonal 1800CT113-128-46 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Human fetal gut, colon, liver or heart.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Neurogenin 3 (NEUROG3)
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别名
- NEUROG3
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背景
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ATH4B, Atoh5, bHLHa7, Class A basic helix-loop-helix protein 7, Math4B, NEUROG3, Neurogenin-3, Neurogenin3, NGN-3, ngn3, Protein atonal homolog 5,NEUROG3 / Neurogenin 3 / NGN3 (Transcription Factor)
Cellular localisation: Nucleus. -
分子量
- 23.54kDa
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基因ID
- 50674, 288655
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UniProt
- Q9Y4Z2
抗原
-
