Recombinant MYOD1 抗体 (AA 1-100)
Quick Overview for Recombinant MYOD1 抗体 (AA 1-100) (ABIN7126737)
抗原
See all MYOD1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-100
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特异性
- MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase, thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS). RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors. Studies suggest, anti-MyoD1 may be used together with anti-Myogenin and anti-Desmin as a panel of markers since any RMS is virtually never negative for all three markers simultaneously.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa1-100) of human MyoD1 protein (exact sequence is proprietary)
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亚型
- IgG
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anti-Myogenic Differentiation 1 (MYOD1) (AA 150-250) antibody
MYOD1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 51-150) antibodyMYOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 兔 Monoclonal MYOD1-2075R unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rMYD712 unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-240) antibodyMYOD1 适用: 人 WB, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human fetal skeletal muscle or rhabdomyosarcoma.
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限制
- 仅限研究用
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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别名
- MYOD1
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背景
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BHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM,MyoD1 (Rhabdomyosarcoma Marker)
Cellular localisation: Nuclear -
分子量
- 45kDa
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基因ID
- 4654, 181768
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UniProt
- P15172
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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