Recombinant MYH11 抗体 (AA 1-100)
Quick Overview for Recombinant MYH11 抗体 (AA 1-100) (ABIN7126733)
抗原
See all MYH11 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-100
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特异性
- Smooth Muscle Myosin, heavy chain (SMM-HC) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early and is specific for smooth muscle development. SM-MHC stains the intact myoepithelial cell (MEC) layers present in benign and in situ malignant breast and bronchioloalveolar lesions and is therefore very helpful in distinguishing between benign and malignant tumors. The antibody reacts with smooth muscle cells and myoepithelial cells, but not with myofibroblasts. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human fragment corresponding to Myosin residues within aa1-100 of Myosin (Smooth Muscle)
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亚型
- IgG
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human smooth muscle, uterus or breast.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
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别名
- MYH11
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背景
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AAT4, Myosin heavy chain 11 (MYH11), Myosin heavy chain 11 smooth muscle, Smooth muscle myosin heavy chain 11, Myosin-11, SM1, SM2, SMHC, SMMHC,Smooth MuscleMyosin Heavy Chain (SM-MHC) (Leiomyosarcoma & Myoepithelial Cell Marker)
Cellular localisation: Cytoplasm. -
分子量
- 227kDa
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基因ID
- 4629, 460109
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UniProt
- P35749
抗原
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