MITF 抗体
Quick Overview for MITF 抗体 (ABIN7126695)
抗原
See all MITF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.
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交叉反应 （详细）
- Human. Predicted to react in Chick, Mouse, Rat, Xenopus and Zebrafish.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human MITF protein
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亚型
- IgG2b
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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibodyMITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibodyMITF 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 8F1G5 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-28), (N-Term) antibodyMITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibodyMITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated
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应用备注
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Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), Immunofluorescence (1-2 μg/mL),Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells.Human melanoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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别名
- MITF
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背景
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BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A,Microphthalmia Transcription Factor (MITF)
Cellular localisation: Nucleus. -
分子量
- 52-56kDa (doublet)
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基因ID
- 4286
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UniProt
- O75030
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途径
- Chromatin Binding
抗原
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