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Recombinant EpCAM 抗体 (Cytoplasmic Domain)

This anti-EpCAM antibody is a 兔 单克隆 antibody detecting EpCAM in IHC (f). Suitable for 人.
产品编号 ABIN7126663
发货至: 中国

Quick Overview for Recombinant EpCAM 抗体 (Cytoplasmic Domain) (ABIN7126663)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

抗体类型

Recombinant Antibody

适用

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克隆类型

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单克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
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    AA 289-314, Cytoplasmic Domain

    特异性

    Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    交叉反应 (详细)

    Human.Dog. Cat.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment from cytoplasmic domain (around aa 289-314) of human EpCAM (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7, HT29 cells. Human colon or breast.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    TACSTD1

    背景

    Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1),EpCAM / CD326 (Cytoplasmic Domain) (Epithelial Marker)
    Cellular localisation: Cell surface

    分子量

    40-43kDa

    基因ID

    4072, 542050

    UniProt

    P16422
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