Leptin Receptor 抗体 (AA 335-425)
Quick Overview for Leptin Receptor 抗体 (AA 335-425) (ABIN7126638)
抗原
See all Leptin Receptor (LEPR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 335-425
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特异性
- Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84 % identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor. Ob-R mRNA is expressed in the choroid plexus and hypothalamus.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa335-425) of human LEPR protein (exact sequence is proprietary)
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亚型
- IgG1
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anti-Leptin Receptor (LEPR) (AA 901-1000) antibody
LEPR 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 310-539) antibodyLEPR 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 22-203) antibodyLEPR 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 238-375) antibodyLEPR 适用: 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (Internal Region) antibodyVerified LEPR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) antibodyLEPR 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 23-52) antibodyLEPR 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 501-600) antibodyLEPR 适用: 人, 小鼠, 大鼠 WB, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (C-Term) antibodyLEPR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Leptin Receptor (LEPR) (AA 31-130) antibodyLEPR 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H5 unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 or HeLa cells. Human heart, small intestine or fetal liver.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Leptin Receptor (LEPR)
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别名
- LEPR
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背景
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Db, Fa, HuB219, LEP-R, LEPR, LEPRD, Leptin receptor, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein (OB-RGRP), OB receptor (OBR / OB-R), obl,Leptin Receptor (LEPR) / Ob-R / CD295
Cellular localisation: Secreted. Cell surface. -
分子量
- 100kDa / 125kDa (short / long form)
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基因ID
- 3953
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UniProt
- P48357
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途径
- JAK/STAT Signaling, AMPK Signaling, Feeding Behaviour
抗原
-
