Recombinant KRT8 抗体
Quick Overview for Recombinant KRT8 抗体 (ABIN7126623)
抗原
See all KRT8 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- Epitope of this MAb is located between aa343-357. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.
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交叉反应 （详细）
- Human. Does not react with rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Keratin preparation from a human carcinoma
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亚型
- IgG
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anti-Keratin 8 (KRT8) antibody
KRT8 适用: 人, 小鼠 WB, IHC, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal M20 unconjugated
anti-Keratin 8 (KRT8) (AA 92-393) antibodyKRT8 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (Ser726), (Ser731) antibodyKRT8 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) antibodyKRT8 适用: 人, 大鼠, 斑马鱼 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal H1 unconjugated
anti-Keratin 8 (KRT8) (pSer432) antibodyKRT8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) (AA 91-381) antibodyKRT8 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Keratin 8 (KRT8) (AA 1-483) antibodyKRT8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 8 (KRT8) antibodyKRT8 适用: 人, Cow, 兔, Pig, 绵羊 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal B22-1 unconjugated
anti-Keratin 8 (KRT8) (AA 391-483) antibodyKRT8 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8A5D12 unconjugated
anti-Keratin 8 (KRT8) (Middle Region) antibodyKRT8 适用: 人, 大鼠, 小鼠, Cow, 兔, Pig, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HCT116, MCF-7, HeLa or A431 cells. Human skin, colon, lung or breast carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- KRT8 (Keratin 8 (KRT8))
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别名
- KRT8
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背景
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CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 8 (KRT8)
Cellular localisation: Cytoplasmic -
分子量
- 52.5kDa
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基因ID
- 3856
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UniProt
- P05787
抗原
-
