Keratin 6B 抗体
Quick Overview for Keratin 6B 抗体 (ABIN7126618)
抗原
See all Keratin 6B (KRT6B) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
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交叉反应 （详细）
- Human and Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human KRT6B protein
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亚型
- IgG2a
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anti-Keratin 6B (KRT6B) (AA 1-564) antibody
KRT6B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G9 unconjugated
anti-Keratin 6B (KRT6B) (AA 1-280) antibodyKRT6B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 6B (KRT6B) (AA 1-564) antibodyKRT6B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Keratin 6B (KRT6B) (AA 207-236) antibodyKRT6B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33708 unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil or basal cell carcinoma
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Keratin 6B (KRT6B)
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别名
- KRT6B
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背景
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CK6B, Keratin Type II Cytoskeletal 6B, KRT6B,Cytokeratin 6B (KRT6B) (Basal Cell Marker)
Cellular localisation: Cytoplasmic -
分子量
- 56kDa
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基因ID
- 3854, 700779
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UniProt
- P04259
抗原
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