Recombinant Liver Arginase 抗体 (AA 200-322)
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Quick Overview for Recombinant Liver Arginase 抗体 (AA 200-322) (ABIN7126610)
抗原
See all Liver Arginase (ARG1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 200-322
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原理
- Recombinant Arginase 1 (Hepatocellular Carcinoma Marker) Antibody
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特异性
- Recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
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交叉反应 (详细)
- Human. Others not known.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant fragment (around aa200-322) of human ARG1 protein (exact sequence is proprietary)
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亚型
- IgG, kappa
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应用备注
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Known_Application: Western Blotting (2-4 μg/mL),Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: 293T cells. Hepatocellular Carcinoma (HCC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
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别名
- ARG1
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背景
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Arginase 1, ARG1, liver-type arginase, type I arginase
293T cells. Human liver or hepatocellular carcinoma (HCC).
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分子量
- 35-38kDa
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基因ID
- 383, 440934
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UniProt
- P05089
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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