PDX1 抗体
Quick Overview for PDX1 抗体 (ABIN7126596)
抗原
See all PDX1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Pancreatic duodenal homeobox-1 protein (PDX-1), also designated Insulin promoter factor (IPF1), Insulin upstream factor 1 (IUF1), somatostatin transactivating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic i²-cells. PDX-1 is a key regulator of pancreatic islet development and Insulin gene transcription in i²-cells. PDX-1 is expressed in all cells at the early stages of development and is mainly restricted to the pancreas and duodenum in adult. HNF-3i², HNF-1i± and SP1 positively regulate the PDX-1 enhancer element. PDX-1 is also regulated by Glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. The increased levels of cAMP, and the resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation of the protein to the nuclei of i²-cells. PDX-1 binds to the sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by the SAPK2 pathway under high glucose concentrations. Mutations in the PDX-1 gene can cause maturity-onset diabetes of the young and pancreatic agenesis. The gene which encodes PDX-1 maps to human chromosome 13q12.2.
-
交叉反应 （详细）
- Human
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant full-length human PDX1 protein
-
亚型
- IgG2
-
-
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-284) antibody
PDX1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibodyPDX1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30) antibodyPDX1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibodyPDX1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-283) antibodyPDX1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 1-30) antibodyPDX1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15204 unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibodyPDX1 适用: 人 WB, FACS, EIA 宿主: 小鼠 Monoclonal 5A5 unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 188-284) antibodyPDX1 适用: 大鼠, 山羊 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 136-169) antibodyPDX1 适用: 小鼠 WB, IF 宿主: 兔 Polyclonal RB51197 unconjugated
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) (AA 109-208) antibodyPDX1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4E12 unconjugated
-
-
应用备注
-
Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or HEK293 cells.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))
-
别名
- PDX1
-
背景
-
Glucose sensitive factor, GSF, IDX1, Insulin promoter factor 1 homeodomain transcription factor, IPF1, STF1, IUF1, Islet/duodenum homeobox 1, MODY4, Pancreas/duodenum homeobox, Somatostatin transactivating factor 1,Pancreatic duodenal homeobox-1 protein (PDX1)
Cellular localisation: Nucleus. -
分子量
- 46kDa
-
基因ID
- 3651, 32938
-
UniProt
- P52945
-
途径
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location
抗原
-
