Recombinant IDH1 抗体 (AA 281-414)
Quick Overview for Recombinant IDH1 抗体 (AA 281-414) (ABIN7126537)
抗原
See all IDH1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 281-414
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特异性
- IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)
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亚型
- IgG
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anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) antibody
IDH1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 74-333) antibodyIDH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7G8A1 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (N-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (Center) antibodyIDH1 适用: 人, 小鼠 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 156-298) antibodyIDH1 适用: 人, 小鼠, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4A4A8 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 381-413), (C-Term) antibodyIDH1 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB18238 unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 63-90) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 116-143) antibodyIDH1 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, HeLa, HT29 or MCF7cells. Humanbreast, colon or prostate carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
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别名
- IDH1
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背景
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Cytosolic NADP-isocitrate dehydrogenase, Epididymis luminal protein 216, Epididymis secretory protein Li 26, HEL-216, HEL-S-26, ICDH, IDCD, IDH1, IDP, IDPC, Isocitrate dehydrogenase 1 (NADP+) soluble, NADP dependent isocitrate dehydrogenase peroxisomal, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD,IDH1-R132H (Isocitrate Dehydrogenase)
Cellular localisation: Cytoplasm. Nucleus. -
分子量
- 45-47kDa
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基因ID
- 3417, 593422
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UniProt
- O75874
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途径
- Warburg Effect
抗原
-
