GTF2B 抗体
Quick Overview for GTF2B 抗体 (ABIN7126496)
抗原
See all GTF2B 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH, and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the TATA element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human GTF2Bprotein
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亚型
- IgG2b
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anti-General Transcription Factor IIB (GTF2B) antibody
GTF2B 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 50-150) antibodyGTF2B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 1-316) antibodyGTF2B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 1-316) antibodyGTF2B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (N-Term) antibodyGTF2B 适用: 人, 小鼠, 犬, 斑马鱼, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 129-178) antibodyGTF2B 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠, 猴, Pig, 兔, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) antibodyGTF2B 适用: 人, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 4C1 unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 141-250) antibodyGTF2B 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) antibodyGTF2B 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIB (GTF2B) (AA 89-138) antibodyGTF2B 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, 马, Cow, 豚鼠, 猴, Pig, 兔, 小鸡, 非洲爪蟾, Bat WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (1-2 μg/million cells), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or MCF-7 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- GTF2B (General Transcription Factor IIB (GTF2B))
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别名
- GTF2B
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背景
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General transcription factor IIB, General transcription factor TFIIB, gtf2b, RNA polymerase II transcription factor IIB, S300 II, S300-II, TF IIB, TF2B, TFIIB, Transcription initiation factor IIB,GTF2B/ TFIIB (Transcription Factor 2B)
Cellular localisation: Nucleus. Nucleoplasm, nuclear bodies. -
分子量
- 34.8kDa
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基因ID
- 2959, 481852
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UniProt
- Q00403
抗原
-
