GCLM 抗体
Quick Overview for GCLM 抗体 (ABIN7126478)
抗原
See all GCLM 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length GCLM protein
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亚型
- IgG2a
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))
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别名
- GCLM
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背景
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GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody,Glutamate-Cysteine Ligase Regulatory Subunit
Cellular localisation: Cytoplasm. -
分子量
- 31kDa
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基因ID
- 2730, 315562
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UniProt
- P48507
抗原
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