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GCLM 抗体 (Regulatory Subunit)

This 小鼠 单克隆 antibody specifically detects GCLM in WB 和 IHC. It exhibits reactivity toward 人.
产品编号 ABIN7126478
发货至: 中国
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Quick Overview for GCLM 抗体 (Regulatory Subunit) (ABIN7126478)

抗原

See all GCLM 抗体
GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

适用

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宿主

  • 75
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小鼠

克隆类型

  • 47
  • 38
单克隆

标记

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This GCLM antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

CPTC-GCLM-1
  • 抗原表位

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    Regulatory Subunit

    原理

    Glutamate-Cysteine Ligase Regulatory Subunit Antibody

    特异性

    GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

    交叉反应 (详细)

    Human.

    纯化方法

    200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

    免疫原

    Recombinant human full-length GCLM protein

    亚型

    IgG2a, kappa
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10  mM PBS

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    有效期

    24 months
  • 抗原

    GCLM (Glutamate-Cysteine Ligase, Modifier Subunit (GCLM))

    别名

    GCLM

    背景

    GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody

    A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.

    分子量

    31kDa

    基因ID

    2730, 315562

    UniProt

    P48507
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