CNOT10 抗体
Quick Overview for CNOT10 抗体 (ABIN7126458)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- CNOT10 is a subunit of the CCR4-NOT complex which consists of at least five other CNOT subunit proteins and TAB182. The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription, as well as in mRNA degradation. Various subunits (e.g. CNOT1, CNOT3) are uniquely involved in influencing nuclear hormone receptor activities. In effect, this complex has an important role as a transcription regulator and repressor of nuclear receptor signaling that is relevant to the molecular pathways involved in cancer. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human CNOT10 protein
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亚型
- IgG2
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anti-CCR4-NOT Transcription Complex, Subunit 10 (CNOT10) antibody
CNOT10 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CCR4-NOT Transcription Complex, Subunit 10 (CNOT10) (Internal Region) antibodyCNOT10 适用: 人, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CCR4-NOT Transcription Complex, Subunit 10 (CNOT10) (AA 1-744) antibodyCNOT10 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-CCR4-NOT Transcription Complex, Subunit 10 (CNOT10) (C-Term) antibodyCNOT10 适用: 人, 小鼠, 大鼠, 马, Cow, 犬, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or HepG2 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CNOT10 (CCR4-NOT Transcription Complex, Subunit 10 (CNOT10))
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别名
- CNOT10
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背景
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FLJ12890, FLJ13165,CCR4-NOT transcription complex, subunit 10 (CNOT10)
Cellular localisation: Cytoplasm. Mitochondrion and Nucleus, PML body. -
分子量
- 82.31kDa
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基因ID
- 25904, 444851
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UniProt
- Q9H9A5
抗原
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