SIRT3 抗体
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北京 101111
Quick Overview for SIRT3 抗体 (ABIN7126428)
抗原
See all SIRT3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Sirtuin 3 (SIRT3) Antibody
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特异性
- Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).
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交叉反应 (详细)
- Human.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human SIRT3 protein
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亚型
- IgG2a
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- SIRT3 (Sirtuin 3 (SIRT3))
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别名
- SIRT3
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背景
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hSIRT3, Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase, NAD dependent deacetylase sirtuin 3 mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3, SIR2-like protein 3, SIR2L3, Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae), Sirtuin type 3, Sirtuin3
HeLa cells.
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分子量
- 43.57kDa
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基因ID
- 23410, 716456
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UniProt
- Q9NTG7
抗原
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