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FOXL1 抗体 (AA 104-258)

The 小鼠 单克隆 anti-FOXL1 antibody (ABIN7126396) specifically detects FOXL1 in WB, IF, FACS, IHC (f) 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN7126396
发货至: 中国
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Quick Overview for FOXL1 抗体 (AA 104-258) (ABIN7126396)

抗原

See all FOXL1 抗体
FOXL1 (Forkhead Box L1 (FOXL1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This FOXL1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
  • 抗原表位

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    AA 104-258

    特异性

    FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyer's patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.

    交叉反应 (详细)

    Human. Predicted to react in Rat.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa104-258) of human FOXL1 protein (exact sequence is proprietary)

    亚型

    IgG1
  • 应用备注

    Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    FOXL1 (Forkhead Box L1 (FOXL1))

    别名

    FOXL1

    背景

    FKHL10, FREAC6, JFJ-3, FKH6, FKH11, Forkhead box protein L1, Forkhead related transcription factor, FREAC7,FOXL1 / Forkhead Box Protein L1 (Transcription Factor)
    Cellular localisation: Nucleus.

    分子量

    36-41kDa

    基因ID

    2299, 87236

    UniProt

    Q12951
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