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JAZF1 抗体 (AA 1-130)

This 小鼠 单克隆 anti-JAZF1 antibody specifically detects JAZF1 in WB, FACS, IF, IHC (f) 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN7126392
发货至: 中国
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Quick Overview for JAZF1 抗体 (AA 1-130) (ABIN7126392)

抗原

See all JAZF1 抗体
JAZF1 (JAZF Zinc Finger 1 (JAZF1))

适用

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宿主

  • 20
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小鼠

克隆类型

  • 23
单克隆

标记

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This JAZF1 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
  • 抗原表位

    • 4
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    AA 1-130

    特异性

    JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

    交叉反应 (详细)

    Human. Predicted to react in Mouse.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)

    亚型

    IgG2a
  • 应用备注

    Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    JAZF1 (JAZF Zinc Finger 1 (JAZF1))

    别名

    JAZF1

    背景

    DKFZp761K2222, TIP27, ZNF802,JAZF1 (Transcription Corepressor)
    Cellular localisation: Nucleus.

    分子量

    27.08kDa

    基因ID

    221895, 368944

    UniProt

    Q86VZ6
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