Tissue factor 抗体 (AA 32-100)
(7 references)
(1 validation)Quick Overview for Tissue factor 抗体 (AA 32-100) (ABIN708086)
抗原
See all Tissue factor (F3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 32-100
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交叉反应
- 人, 小鼠, Pig, 大鼠
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预测反应
- Dog,Cow,Horse,Rabbit,Guinea Pig
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human CD142
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亚型
- IgG
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anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 45-154) antibody
F3 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 4G4 unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 36-292) antibodyF3 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 45-154) antibodyF3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G4 unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 1-295) antibodyF3 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) antibodyF3 适用: 人 WB, ICC, FACS, IHC (p) 宿主: 小鼠 Monoclonal HTF-1 unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 1-295) antibodyF3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 30-252) antibodyF3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 30-251) antibodyF3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 34-251) antibodyF3 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 34-251) antibodyF3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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- by
- Reveal Biosciences
- No.
- #029589
- 日期
- 2014.02.07
- 抗原
- Lot Number
- 980886W
- Method validated
- Immunohistochemistry
- Positive Control
- Mouse lung tissue
- Negative Control
- Mouse brain tissue
- Notes
- Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
- Primary Antibody
- Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
- Catalog number: ABIN708086
- Lot number: 120224
- Secondary Antibody
- Antibody: Bond Polymer Refine Detection Kit
- Lot number: 24144
- Full Protocol
- Immunohistochemistry was performed on a Leica Bond automated immunostainer.
- Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
- Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
- Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
- Sections were washed x 3 in Leica Bond Wash Buffer.
- Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
- Sections were washed x 3 in Leica Bond Wash Buffer.
- Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
- Sections were washed x 4 in Leica Bond Wash Buffer.
- Sections were washed x 1 in Distilled Water.
- Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
- Sections were washed x 4 in Leica Bond Wash Buffer.
- Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
- Sections were washed x 3 in Distilled Water.
- Sections were counterstained with hematoxylin (Leica) for 2 minutes.
- Sections were washed x 1 in Distilled Water.
- Sections were washed x 1 in Leica Bond Wash Buffer.
- Sections were washed x 1 in Distilled Water.
- Sections were dehydrated, mounted and photographed under a light microscope.
- Experimental Notes
- None notes.
生效 #029589 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Mouse lung tissue stained with anti-F3 (brown) and counterstained in blue.]()
Figure 1: Mouse lung tissue stained with anti-F3 (brown) and counterstained in blue.
![Figure 2: Mouse brain tissue stained with anti-F3 (brown) and counterstained in blue.]()
Figure 2: Mouse brain tissue stained with anti-F3 (brown) and counterstained in blue.
![Figure 3: Mouse lung tissue stained with isotype control antibody (brown) and counterstained in blue.]()
Figure 3: Mouse lung tissue stained with isotype control antibody (brown) and counterstained in blue.
![Figure 4: Mouse lung tissue stained with secondary antibody only (brown) and counterstained in blue.]()
Figure 4: Mouse lung tissue stained with secondary antibody only (brown) and counterstained in blue.
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).
: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).
: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).
: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).
: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).
: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).
: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).
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: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).
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- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
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别名
- CD142
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背景
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Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3
Background: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
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基因ID
- 2152
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UniProt
- P13726
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途径
- Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
-
