RTF1 抗体
Quick Overview for RTF1 抗体 (ABIN7118711)
抗原
See all RTF1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- RTF1 antibody
-
纯化方法
- Immunogen affinity purified
-
纯度
- ≥95 % as determined by SDS-PAGE
-
免疫原
- Rtf1, Paf1/RNA polymerase II complex component, homolog
-
亚型
- IgG
-
-
anti-RNA polymerase-associated protein RTF1 homolog (RTF1) (AA 620-670) antibody
RTF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA polymerase-associated protein RTF1 homolog (RTF1) (AA 300-400) antibodyRTF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB: 1:500-1:2000, IHC: 1:20-1:200
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freeze / thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- -20°C for 12 months
-
有效期
- 12 months
-
-
- RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))
-
别名
- RTF1
-
背景
-
Synonyms: RNA polymerase-associated protein RTF1 homolog|RTF1|KIAA0252
Background: Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4'(H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex(By similarity).
-
分子量
- 100 kDa
-
基因ID
- 23168
-
UniProt
- Q92541
-
途径
- Stem Cell Maintenance
抗原
-
