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Recombinant CD43 抗体

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, WB, IHC, IF 和 IP. Suitable for 人.
产品编号 ABIN7072631
发货至: 中国

Quick Overview for Recombinant CD43 抗体 (ABIN7072631)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

84-3C1
  • 原理

    Anti-CD43 [84-3C1], Mouse IgG2b, kappa

    特异性

    This antibody recognizes CD43 which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration.

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2b

    纯化方法

    Protein A affinity purified

    免疫原

    This antibody was raised by immunizng mice with stimulated human leukocytes.

    亚型

    IgG2b kappa
  • 应用备注

    This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37 % of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells' platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017).

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    CD43

    背景

    Leukosialin, Sialophorin, GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein

    UniProt

    P16150

    途径

    Regulation of Leukocyte Mediated Immunity
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