Recombinant IL2R gamma C 抗体
适用: 小鼠
BP, FACS, IP
宿主: 大鼠
Monoclonal
3E12
unconjugated
Recombinant Antibody
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- 抗原
- IL2R gamma C
- 抗体类型
- Recombinant Antibody
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Blocking Peptide (BP), Flow Cytometry (FACS), Immunoprecipitation (IP)
- 原理
- Anti-IL2R gamma C [3E12], Rat IgG2b, kappa
- 特异性
- This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognises an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2. This antibody does not cross-react with COS7 cells transfected with rat common gamma-chain cDNA (Olosz et al, 2002).
- 产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
- 纯化方法
- Protein A affinity purified
- 免疫原
- This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).
- 克隆位点
- 3E12
- 亚型
- IgG2b kappa
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anti-IL2R gamma C antibody
适用: 小鼠 BP, FACS, IP 宿主: 兔 Chimeric 3E12 unconjugated Recombinant Antibody
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- 应用备注
- This antibody has been used in flow cytometric analysis of common gamma-chain surface expression by IL-7-stimulated T cells (Goh et al, 2017) and COS7 monkey kidney cells (Olosz et al, 2002). This antibody has also been used to immunoprecipiate murine common gamma-chain-containing complexes from COS7 cell extracts (Olosz et al, 2002). Additionally, as this antibody is conformationally sensitive, it has been used to confirm the correct folding of soluble murine common gamma chain mutants (Meißner et al, 2001). This antagonistic antibody is most effective at inhibiting IL-4 bioactivity (Malek et al, 1998). Treatment of recipient mice with this antibody, along with another common gamma-chain-blocking MAb (4G3), significantly prolongs islet allograft survival (Li et al, 2000). Indeed, blocking the common gamma chain with this antibody has been shown to induce apoptotic cell death of BAF-B03 cells and primary anti-CD3 activated T lymphoblasts in vitro, and of alloreactive T cells in vivo (Li et al, 2000).
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- IL2R gamma C
- 背景
- CD132, IL2R gamma C, common gamma chain, common y chain, gamma chain, IL-2 receptor gamma chain, Interleukin-2 receptor subunit gamma, Cytokine receptor common subunit gamma, Il2rg, IL-2 receptor subunit gamma, IL-2R subunit gamma, IL-2RG, gammaC, p64
- UniProt
- P34902
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