Recombinant LY6C + LY6G 抗体
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- 抗原
- LY6C + LY6G
- 抗体类型
- Recombinant Antibody
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Depletion (Dep)
- 原理
- Anti-Ly6G/Ly6C [RB6-8C5], Rabbit IgG, kappa
- 特异性
- This antibody is specific for murine Ly6G, a a 21-25 kDa glycosylphosphatidylinositol (GPI)-linked protein expressed by the myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during bone marrow development, while Ly6G expression in granulocytes and peripheral neutrophils directly correlates with the cell's level of differentiation and maturation. This hallmark makes Ly6G a good marker for these particular cell populations. Ly6G has also been implicated in the development of antitumor responses.
- 产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
- 纯化方法
- Protein A affinity purified
- 免疫原
- This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
- 克隆位点
- RB6-8C5
- 亚型
- IgG kappa
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anti-LY6C + LY6G antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE
anti-LY6C + LY6G antibody适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated
anti-LY6C + LY6G antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC
anti-LY6C + LY6G antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7
anti-LY6C + LY6G antibody (SPRD)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 SPRD
anti-LY6C + LY6G antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Biotin
anti-LY6C + LY6G antibody (FITC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC
anti-LY6C + LY6G antibody (APC-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC-Cy7
anti-LY6C + LY6G antibody (Cy5)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Cy5
anti-LY6C + LY6G antibody (PE-Cy5.5)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy5.5
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- 应用备注
- This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to elucidate the origin and roles of macrophages in the post-myocardial infarction remodelling (Sager et al, 2016), to investigate the pathogenesis of primary biliary cirrhosis (Yang et al, 2016), to confirm the phenotypic dynamics of microglial and monocyte-derived cells in glioblastoma-bearing mice (Ricard et al, 2016), and to highlight an important ageing-related defect in the mucosal immune system's ability to sample lumenal antigens (Kobayashi et al, 2013). This antibody has also been used in vivo, for example, to deplete Treg cells when delineating the paralleled developments of inflammation and immunosuppression in tumor microenvironment (Yang et al, 2010), or to deplete neutrophils when underpinning a causal role for neutrophils in severe asthma (Wilson et al, 2009).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- LY6C + LY6G
- 别名
- Ly6G/Ly6C
- 背景
- Gr-1, Gr1, Ly-6G, ly-6G.1, ly6C
- UniProt
- P35461
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