Recombinant CD131 抗体
Quick Overview for Recombinant CD131 抗体 (ABIN7072453)
抗原
See all CD131 (CSF2RB) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD131 [BION-1], Mouse IgG1, kappa
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特异性
- Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
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亚型
- IgG1 kappa
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anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 17-149) antibody
CSF2RB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1D5E4 unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 133-233) antibodyCSF2RB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 17-149) antibodyCSF2RB 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 8H8D7 unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 492-710) antibodyCSF2RB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) antibodyCSF2RB 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (C-Term) antibodyCSF2RB 适用: 人, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 1-889) antibodyCSF2RB 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (AA 559-608) antibodyCSF2RB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) (Center) antibodyCSF2RB 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Beta (CSF2RB) antibodyCSF2RB 适用: 人 WB, IP, FACS 宿主: 小鼠 Monoclonal 1C1 unconjugated
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应用备注
- BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun et al., 1999, PMID: 10477723). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom et al., 2002, PMID: 12444058). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn et al., 2000, PMID: 10753826).
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说明
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This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
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别名
- CD131
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背景
- CSF2RB, CD131, IL3RB, IL-3R common beta subunit, IL5RB, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage), SMDP5, Common Beta-Chain, CDw131, cytokine receptor common subunit beta, beta-c, CD131, Beta Common Chain
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UniProt
- P32927
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途径
- JAK/STAT Signaling
抗原
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