Recombinant Hemoglobin 抗体
Quick Overview for Recombinant Hemoglobin 抗体 (ABIN7072452)
抗原
See all Hemoglobin 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Haemoglobin [2A1], Rabbit IgG, kappa
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特异性
- 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.
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亚型
- IgG kappa
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anti-Hemoglobin antibody
适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: 猕猴 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody (APC)适用: 兔 WB, IHC, ICC, IP 宿主: 豚鼠 Polyclonal APC
anti-Hemoglobin antibody适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 4A1B1 unconjugated
anti-Hemoglobin antibody适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: 豚鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hemoglobin antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- 2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Hemoglobin
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别名
- Haemoglobin
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背景
- Hemoglobin, Hb
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UniProt
- P01942
抗原
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