Recombinant T Cell Receptor 抗体
-
- 抗原
- T Cell Receptor
- 抗体类型
- Recombinant Antibody
-
适用
- 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunofluorescence (IF)
- 原理
- Anti-TCR [Desire-1], Mouse IgG1, kappa
- 特异性
- MAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
- 产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG
- 纯化方法
- Protein A affinity purified
- 免疫原
- mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
- 克隆位点
- Desire-1
- 亚型
- IgG1 kappa
-
-
- 应用备注
- Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).
- 说明
-
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
-
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-