Recombinant MHC, Class I 抗体
Quick Overview for Recombinant MHC, Class I 抗体 (ABIN7072425)
抗原
See all MHC, Class I 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-MHC I [R1-9.6], Rat IgG2a, kappa
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特异性
- This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody recognises a determinant distinct from those detected by most common mouse monoclonal anti-H-2 antibodies.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
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亚型
- IgG2a kappa
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anti-MHC, Class I antibody
适用: 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 绵羊, Cat FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal VPM19 unconjugated
anti-MHC, Class I antibody适用: 小鼠 IHC, IP, IA 宿主: 兔 Chimeric R1-21-2 unconjugated Recombinant Antibody
anti-MHC, Class I (AA 81-180) antibody适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-MHC, Class I antibody适用: 小鼠 IP, IF, IA 宿主: 兔 Chimeric R1-9-6 unconjugated Recombinant Antibody
anti-MHC, Class I antibody (PE)适用: 小鸡 FACS, IHC 宿主: 小鼠 Monoclonal F21-2 PE
anti-MHC, Class I antibody适用: 小鸡 FACS, WB, IHC, IP 宿主: 小鼠 Monoclonal F21-2 unconjugated
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应用备注
- This antibody binds murine H-2 MHC class I molecules and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987) and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al., 2004) and MHC-I endocytosis (Knorr et al., 2009). This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al., 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells and assess its strain specificity (Koch et al., 1983, PMID: 6192569).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- MHC, Class I
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别名
- MHC I
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背景
- R1.9.6, H-2, MHC-I, MHC Class I
抗原
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