Recombinant MHC Class II 抗体
Quick Overview for Recombinant MHC Class II 抗体 (ABIN7072422)
抗原
See all MHC Class II (MHC2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-MHC II [P7/7], Rabbit IgG, kappa
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特异性
- This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
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交叉反应
- 小鼠
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
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亚型
- IgG kappa
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anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, Func, IHC (p) 宿主: 大鼠 Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 人, Cow, 绵羊, 山羊 FACS, IHC, IHC (p) 宿主: 小鼠 Monoclonal FITC
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应用备注
- This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
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别名
- MHC II
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背景
- P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
抗原
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