Recombinant LTA 抗体
Quick Overview for Recombinant LTA 抗体 (ABIN7072400)
抗原
See all LTA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Lymphotoxin alpha [3F12.2D3], Mouse IgG2b, kappa
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特异性
- 3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE, ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
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亚型
- IgG2b kappa
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anti-Lymphotoxin-alpha (LTA) (AA 49-202) antibody
LTA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 36-205) antibodyLTA 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 39-281) antibodyLTA 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (Internal Region) antibodyLTA 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 1-205) antibodyLTA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 36-205) antibodyLTA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 35-191) antibodyLTA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 46-72) antibodyLTA 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21239 unconjugated
anti-Lymphotoxin-alpha (LTA) (Middle Region) antibodyLTA 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Lymphotoxin-alpha (LTA) (AA 116-203) antibodyLTA 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- LTA (Lymphotoxin-alpha (LTA))
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别名
- Lymphotoxin alpha
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物质类
- Chemical
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背景
- LTα3 chimeric 3F12,LT, LTα, Tumor necrosis factor beta, Tumor necrosis factor β, Tumor necrosis factor-beta, tumor necrosis factor-β, TNF beta, TNF-beta, TNF β, TNF-β, Lymphotoxin-alpha, Lymphotoxin alpha
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UniProt
- P01374
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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