Recombinant Fc gamma RII (CD32) 抗体
Quick Overview for Recombinant Fc gamma RII (CD32) 抗体 (ABIN7072239)
抗原
See all Fc gamma RII (CD32) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD32 [AT130-5], Rabbit IgG, kappa
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特异性
- This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c FcgammaRIIB-/- mice with FcgammaRIIB-rat CD4 domain 3/4 fusion protein.
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亚型
- IgG kappa
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anti-Fc gamma RII (CD32) antibody
CD32 适用: 人 FACS, WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6D11 unconjugated
anti-Fc gamma RII (CD32) (AA 201-300) antibodyCD32 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 PE
anti-Fc gamma RII (CD32) (AA 69-309) antibodyCD32 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibody (PE)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 PE
anti-Fc gamma RII (CD32) antibody (FITC)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 FITC
anti-Fc gamma RII (CD32) (AA 36-229) antibodyCD32 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人, 犬, 猕猴, Pig FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal AT10 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: Cow, 绵羊 FACS 宿主: 小鼠 Monoclonal CCG36 PE
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应用备注
- The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011, PMID: 21742972), and that immunotherapy targeting murine FcγRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013, PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcγRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcγRs (Vollack et al., 2017, PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017, PMID: 28492697).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Fc gamma RII (CD32)
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别名
- CD32
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背景
- FcγRIIB, FcgRIIb, CD32B, CDw32, FCG2, FCGR2, FCGR2B, IGFR2, Immunoglobulin G Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II-b, MGC23887, MGC30032, RP11-5K23.6
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UniProt
- P08101
抗原
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