SNAP25 抗体 (Cytosolic)
Quick Overview for SNAP25 抗体 (Cytosolic) (ABIN7043792)
抗原
See all SNAP25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cytosolic
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原理
- A Rabbit Polyclonal Antibody to SNAP-25
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特异性
- Cytoplasmic
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Human,mouse - identical
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产品特性
- Anti- Antibody is directed against rat . Anti- Antibody (ABIN7043792 and ABIN7044647) can be used in western blot and immunohistochemical applications. It has been designed to recognize from rat, mouse and human samples.
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纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
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免疫原
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Immunogen: GST fusion protein
Immunogen Sequence: GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25
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亚型
- IgG
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anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibody
SNAP25 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 1-206) antibodySNAP25 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibodyVerified SNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 166-206) antibodySNAP25 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 192-206) antibodySNAP25 适用: 人, 大鼠, 小鼠, 小鸡, 斑马鱼, Hamster, Goldfish WB, ELISA, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (AA 31-130) antibodySNAP25 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A3 unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) (C-Term) antibodySNAP25 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptosomal-Associated Protein, 25kDa (SNAP25) antibodySNAP25 适用: 人, 大鼠, 小鼠, Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:1000
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说明
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Cited Application: IHC
Negative Control: (ABIN7237327)
Blocking Peptide: (ABIN7237327)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
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别名
- SNAP25
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背景
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Synaptosomal-associated protein 25 kD,SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2.SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.SNAP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.
Alternative names: SNAP-25, Synaptosomal-associated protein 25 kD -
基因ID
- 25012
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NCBI登录号
- NM_130811
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UniProt
- P60881
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途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
抗原
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