Synaptophysin 抗体 (Intravesicular Loop)
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Quick Overview for Synaptophysin 抗体 (Intravesicular Loop) (ABIN7043791)
抗原
See all Synaptophysin (SYP) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 178-190, Intravesicular Loop
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原理
- A Rabbit Polyclonal Antibody to Synaptophysin
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat,mouse,human - 12,13 amino acid residues identical
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产品特性
- Anti-Synaptophysin Antibody (ABIN7043791, ABIN7044656 and ABIN7044657) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptophysin from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin
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亚型
- IgG
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anti-Synaptophysin (SYP) (AA 150-250) antibody
SYP 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (C-Term) antibodySYP 适用: 人 WB, IF, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibodySYP 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB32966 unconjugated
anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibodySYP 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB32966 unconjugated
anti-Synaptophysin (SYP) (N-Term) antibodySYP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) antibodySYP 适用: 人, 犬 WB, IF 宿主: 小鼠 Monoclonal 5H2 unconjugated
anti-Synaptophysin (SYP) (Internal Region) antibodySYP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (N-Term) antibodySYP 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (AA 1-313) antibodySYP 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated
anti-Synaptophysin (SYP) antibodySYP 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3E2 unconjugated
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应用备注
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WB: 1:400
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:100
IP: The optimal concentration should be determined by the user
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说明
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Cited Application: IP|IHC
Negative Control: (ABIN7237323)
Blocking Peptide: (ABIN7237323)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- Synaptophysin (SYP)
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别名
- SYP
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背景
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Synonyms: Major synaptic vesicle protein p38, SYP
Description: Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.
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基因ID
- 24804
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NCBI登录号
- NM_003179
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UniProt
- P07825
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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