Synaptophysin 抗体 (IntravesicularAA 178-190)
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Quick Overview for Synaptophysin 抗体 (IntravesicularAA 178-190) (ABIN7043790)
抗原
See all Synaptophysin (SYP) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- IntravesicularAA 178-190
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原理
- A Guinea Pig Polyclonal Antibody to Synaptophysin
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特异性
- 2nd intravesicular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse,human - 12 out of 13 amino acid residues identical
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产品特性
- A Guinea Pig Polyclonal Antibody to Synaptophysin.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin
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亚型
- IgG
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anti-Synaptophysin (SYP) (AA 150-250) antibody
SYP 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (C-Term) antibodySYP 适用: 人 WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibodySYP 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB32966 unconjugated
anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibodySYP 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB32966 unconjugated
anti-Synaptophysin (SYP) (Internal Region) antibodySYP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) antibodySYP 适用: 人, 犬 WB, IF 宿主: 小鼠 Monoclonal 5H2 unconjugated
anti-Synaptophysin (SYP) (N-Term) antibodySYP 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Synaptophysin (SYP) (AA 274-313) antibodySYP 适用: 人 IHC 宿主: 兔 Monoclonal 462R unconjugated Recombinant Antibody
anti-Synaptophysin (SYP) antibodyMicroarray verified SYP 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB551901 unconjugated
anti-Synaptophysin (SYP) (AA 1-313) antibodySYP 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7237323)
Blocking Peptide: (ABIN7237323)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Synaptophysin (SYP)
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别名
- SYP
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背景
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Major synaptic vesicle protein p38, SYP,Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.
Alternative names: Major synaptic vesicle protein p38, SYP -
基因ID
- 24804
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NCBI登录号
- NM_003179
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UniProt
- P07825
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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