STIM1 抗体 (Extracellular)
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- 抗原 See all STIM1 抗体
- STIM1 (Stromal Interaction Molecule 1 (STIM1))
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抗原表位
- AA 56-69, Extracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This STIM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to STIM1
- 特异性
- STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat,mouse,bovine,canis - identical
- 产品特性
- Anti-STIM1 (extracellular) Antibody (ABIN7043781, ABIN7044026 and ABIN7044027) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 质量等级
- KO Validated
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1
- 亚型
- IgG
- Top Product
- Discover our top product STIM1 Primary Antibody
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anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-685) antibody
STIM1 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 1-211) antibodySTIM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal CDN3H4 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, IHC (p), IF, EIA 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 24-686) antibodySTIM1 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 5A2 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538), (C-Term) antibodySTIM1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB19222 unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (Middle Region) antibodySTIM1 适用: 人, 大鼠, 小鼠, 犬, Cow, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 509-538) antibodySTIM1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibodySTIM1 适用: 人, 大鼠, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Stromal Interaction Molecule 1 (STIM1) (AA 85-180) antibodySTIM1 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:1000
- 说明
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Negative Control: (ABIN7237292)
Blocking Peptide: (ABIN7237292)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- STIM1 (Stromal Interaction Molecule 1 (STIM1))
- 别名
- STIM1 (STIM1 产品)
- 背景
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Stromal interaction molecule 1, GOK,Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 Molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.
Alternative names: STIM1, Stromal interaction molecule 1, GOK - 基因ID
- 6786
- NCBI登录号
- NM_003156
- UniProt
- Q13586
- 途径
- TCR Signaling, BCR Signaling
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