SLC5A1 抗体 (Extracellular)
Quick Overview for SLC5A1 抗体 (Extracellular) (ABIN7043735)
抗原
See all SLC5A1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 13-25, Extracellular
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原理
- A Rabbit Polyclonal Antibody to SGLT1 Transporter
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特异性
- Extracellular, N-terminus
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Not recommended for human samples.
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预测反应
- Mouse - 12,13 amino acid residues identical
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产品特性
- Anti-SGLT1 (extracellular) Antibody (ABIN7043735, ABIN7044491 and ABIN7044492) is a highly specific antibody directed against an epitope of the rat sodium/glucose cotransporter 1. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize SGLT1 from mouse and rat samples. The antibody is not recommended for use on human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)TDAPIQSYERIRN, corresponding to amino acid residues 13-25 of rat SGLT1
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亚型
- IgG
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anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 401-500) antibody
SLC5A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (Internal Region) antibodyVerified SLC5A1 适用: 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 564-643) antibodySLC5A1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (Internal Region) antibodyVerified SLC5A1 适用: 人 WB, ELISA, IHC, ICC 宿主: 山羊 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 239-288) antibodySLC5A1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 1-664) antibodySLC5A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 1-664) antibodySLC5A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (Center) antibodySLC5A1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1) (AA 350-450) antibodySLC5A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IHC
Negative Control: (ABIN7237133)
Blocking Peptide: (ABIN7237133)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))
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别名
- SLC5A1
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背景
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Na+/glucose cotransporter 1, NAGT, High affinity sodium-glucose cotransporter, SLC5A1,The SGLT-1 (Sodium-glucose linked transporter) is part of the SLC5 family of sodium-glucose transporters. This family has more than 220 members in human and bacterial cells and they are expressed in various tissues such as the gut, kidneys and the central nervous system. In human, these transporters are encoded by eleven genes1. SGLT-1 plays a vital role in carbohydrate metabolism and is mainly located in the brush membrane of mature enterocytes in the small intestine. It absorbs D-galactose and D-glucose derived from ingested food2.SGLT-1 has 14 transmembrane α-helices with both the hydrophobic NH2 and hydrophilic COOH termini facing the extracellular side of the cell. SGLT-1 is a symport type transporter carrying sodium and glucose from extracellular space into the cell's cytoplasm. It is believed that sodium binds to the hydrophobic part of the protein and causes a conformational change that enables sugar to binds to the COOH terminal3. The transporter has a number of consensus sites for glycosylation but none are required for the transporter's function.The transporter's regulation was found to be modulated by PKA and PKC. PKA increases vesicle exocytosis 100-fold. Interestingly, the activation of PKC inhibits rat and rabbit SGLT-1 while stimulates human SGLT-1.Pathologies linked to SGLT-1 include the glucose-galactose mal-absorption (GGM) disease caused by a mutation in the transporter. In mice, SGLT-1 knockout causes an inability to survive on glucose containing diets and a decreased insulin and incretin response to glucose4. SGLT-1 is also related to the successful treatment of secretory diarrhea by oral rehydration.
Alternative names: SGLT1, Na+/glucose cotransporter 1, NAGT, High affinity sodium-glucose cotransporter, SLC5A1 -
基因ID
- 25552
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NCBI登录号
- NM_000343
抗原
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