SLC40A1 抗体 (AA 261-275)
Our Local Distributor
北京 101111
Quick Overview for SLC40A1 抗体 (AA 261-275) (ABIN7043729)
抗原
See all SLC40A1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 261-275
-
原理
- A Rabbit Polyclonal Antibody to Ferroportin (SLC40A1)
-
特异性
- 2nd intracellular loop Note: the structure of Ferroportin is still controversial and there is no defined topology yet.
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Mouse,rat - 13,15 amino acid residues identical
-
产品特性
- Anti-Ferroportin (SLC40A1) Antibody is directed against an epitope of human Ferroportin-1. Anti-Ferroportin (SLC40A1) Antibody (ABIN7043729, ABIN7044546 and ABIN7044547) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ferroportin-1 from human, rat, and mouse samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin
-
亚型
- IgG
-
-
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 331-430) antibody
SLC40A1 适用: 人, 小鼠, 小鸡 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 1-571) antibodySLC40A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) antibodySLC40A1 适用: 人, 小鼠, 大鼠, 犬, Pig WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 127-321) antibodySLC40A1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 127-321) antibodySLC40A1 适用: 人 WB 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 245-259) antibodyVerified SLC40A1 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) antibodySLC40A1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 148-298) antibodySLC40A1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 1-571) antibodySLC40A1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:500
-
说明
-
Negative Control: (ABIN7237110)
Blocking Peptide: (ABIN7237110)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 0.2 mL double distilled water (DDW).
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))
-
别名
- SLC40A1
-
背景
-
Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4,Ferroportin (SLC40A1) is an iron transporter suggested to play roles in intestinal iron absorption and cellular iron release. To date, Ferroportin is the only known mammalian iron exporter and is essential for transport of iron from one cell type to another1.Ferroportin has 9-12 transmembrane domains. The topology of the transporter has not been defined with precision and the number of transmembrane domains is still under scrutiny. Studies indicate that the amino terminus of Ferroportin is cytosolic. The location of the carboxyl-terminal, however, is unclear. A second controversial issue regarding Ferroportin structure is whether Ferroportin is a monomer or dimer. Many reports using similar approaches including chromatography, cross-linking and physical techniques such as birefringence, have led to contrasting results2.Ferroportin is found in all the tissues where major iron flows are regulated, including duodenal enterocytes, placental trophoblast, macrophages, and hepatocytes3.Mutations that result in defective Ferroportin transport activity will lead to the phenotype of "classic" Ferroportin disease, which is macrophage iron loading and normal to low transferrin saturation2. Expression of mutant Ferroportin in some cultured cell types results in defects in Ferroportin trafficking where the mutant protein never reaches the cell surface4. In other cell types, the mutant Ferroportin was shown to accumulate at the cell surface but had defective transport activity5.
Alternative names: Ferroportin (SLC40A1), Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4 -
基因ID
- 30061
-
NCBI登录号
- NM_014585
-
UniProt
- Q9NP59
-
途径
- Transition Metal Ion Homeostasis
抗原
-
