RYR1 抗体 (Intracellular)
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- 抗原 See all RYR1 抗体
- RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))
- 抗原表位
- AA 1371-1386, Intracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RYR1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
- 原理
- A Rabbit Polyclonal Antibody to Ryanodine Receptor 1
- 特异性
- Intracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat,mouse - identical
- 产品特性
- Anti-Ryanodine Receptor 1 Antibody (ABIN7043604, ABIN7045211 and ABIN7045212)) is a highly specific antibody directed against an epitope of human RyR1. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR1 from human, rat, and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1
- 亚型
- IgG
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anti-Ryanodine Receptor 1 (Skeletal) (RYR1) antibody
RYR1 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Ryanodine Receptor 1 (Skeletal) (RYR1) (AA 1-534) antibodyRYR1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IP|IHC|IFC
Negative Control: BLP-RR001
Blocking Peptide: BLP-RR001
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))
- 别名
- RYR1 (RYR1 产品)
- 背景
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RyR1, Skeletal muscle-type ryanodine receptor,It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR1 is expressed predominantly in skeletal muscle and in areas of the brain, human RyR1 has at least three known alternative splice variants.Several diseases are attributed to mutations in the RyR1 gene: Central core disease (CCD), multi-minicore disease (MmD) and Malignant hyperthermia (MH) 4.
Alternative names: Ryanodine Receptor 1, RyR1, Skeletal muscle-type ryanodine receptor - 基因ID
- 6261
- NCBI登录号
- NM_000540
- UniProt
- P21817
- 途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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