PRLHR 抗体 (AA 247-260)
Quick Overview for PRLHR 抗体 (AA 247-260) (ABIN7043562)
抗原
See all PRLHR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 247-260
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原理
- A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor
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特异性
- 3rd intracellular loop
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 13,14 amino acid residues identical
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产品特性
- Anti-PrRP Receptor (GPR10) Antibody is directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (ABIN7043562, ABIN7045116 and ABIN7045117) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR
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亚型
- IgG
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anti-Prolactin Releasing Hormone Receptor (PRLHR) (Extracellular Domain) antibody
PRLHR 适用: 人, 大鼠 ELISA, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolactin Releasing Hormone Receptor (PRLHR) (Internal Region) antibodyPRLHR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prolactin Releasing Hormone Receptor (PRLHR) (AA 161-260) antibodyPRLHR 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Prolactin Releasing Hormone Receptor (PRLHR) (Extracellular Domain) antibodyPRLHR 适用: 人, 大鼠, 猴 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolactin Releasing Hormone Receptor (PRLHR) (Extracellular Domain) antibodyPRLHR 适用: 人, Cow, 犬, 马, Pig, 猴 ELISA, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolactin Releasing Hormone Receptor (PRLHR) (AA 1-70) antibodyPRLHR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:1000
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说明
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Negative Control: (ABIN7236617)
Blocking Peptide: (ABIN7236617)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))
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别名
- PRLHR
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背景
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Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR,Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.PrRP is implicated in the regulation of the hypothalamic-pituitary-adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.
Alternative names: PrRP Receptor (GPR10), Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR -
基因ID
- 246075
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NCBI登录号
- NM_004248
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UniProt
- Q64121
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途径
- Feeding Behaviour
抗原
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