KCNMA1 抗体 (Intracellular)
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北京 101111
Quick Overview for KCNMA1 抗体 (Intracellular) (ABIN7043492)
抗原
See all KCNMA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1097-1196, Intracellular
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原理
- A Guinea Pig Polyclonal Antibody to KCNMA1 (KCa1.1) Channel
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特异性
- Intracellular, C-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - 21,21 amino acid residues identical to the canonical sequence (Accession Q08460), rat - 20,21 amino acid residues identical to the canonical sequence (Accession Q62976), human - 19,21 amino acid residues identical to the canonical sequence (Accession Q12791)
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产品特性
- Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody is directed against an epitope of the mouse KCNMA1 channel. Guinea pig Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody , raised in guinea pigs, can be used in western blot, immunoprecipitation, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KCNMA1 from human, mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-KCNMA1 (KCa1.1) (1097-1196) Antibody (ABIN7043493, ABIN7044924 and ABIN7044925) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
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免疫原
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Immunogen: GST fusion protein
Immunogen Sequence: GST fusion protein with the sequence SHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQNATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC, corresponding to residues 1097-1196 of mouse KCNMA1 variant 2
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亚型
- IgG
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anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (C-Term) antibody
KCNMA1 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 兔, 犬, 马, 豚鼠, 绵羊 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1-86) antibodyKCNMA1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 124-467) antibodyKCNMA1 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 199-213), (Extracellular) antibodyKCNMA1 适用: 大鼠 IHC, WB, IF, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1131-1236) antibodyKCNMA1 适用: 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1080-1129) antibodyKCNMA1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 豚鼠, 绵羊, 小鸡, 非洲爪蟾, 猴, Pig, Bat IHC, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (Internal Region) antibodyKCNMA1 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 兔, 犬, 小鸡, 非洲爪蟾, 猴, Pig, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 1184-1200), (Intracellular) antibodyKO Validated KCNMA1 适用: 小鼠 IHC, WB, IP, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (Internal Region) antibodyKCNMA1 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1) (AA 118-152) antibodyKCNMA1 适用: 人 WB 宿主: 兔 Polyclonal RB53642 unconjugated
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应用备注
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Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))
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别名
- KCNMA1
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背景
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Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1,High conductance voltage and Ca2+-activated K+ channels (also known as BK or Slo1 channels) are a family of K+ channels involved in various physiological processes including neuronal excitability and smooth muscle contraction.KCa1.1 channel, also known as KCNMA1, is a member of the BK family and like other BK channels it is comprised of four identical subunits. Each unit consists of seven transmembrane segments and a large intracellular C-terminus. Transmembrane segments S1 to S4 form the channel's voltage sensor, S5, S6 and the intervening amino acids form the pore and selectivity filter. The C-terminus forms a large cytoplasmic domain. This large domain accounts for two-thirds of the entire channel and is believed to contain the domain for Ca2+ binding. Two tandem C-terminal regulator of K+ conductance (RCK) domains from each of four channel subunits form a gating ring at the intracellular membrane surface. A sequence of amino acids that is known as the Ca2+ bowl, and is located within the second of the tandem RCK domains, creates four Ca2+ binding sites on the outer perimeter of the gating ring between RCK domains.KCa1.1 is regulated, both by membrane depolarization and increased intracellular Ca2+ levels which cause the channel to open. This causes subsequent membrane hyperpolarization that closes voltage- and Ca2+-gated channels thus making KCa1.1 a negative feedback regulator for electrical excitation1.KCa1.1 is implicated in the pathogenesis of several diseases. Elevated levels of KCa1.1 mRNA are detected in malignant pleural mesothelioma patients compared with control patients2. Also, blocking KCa1.1 reduces the symptoms of fibroblast-like synoviocytes in rheumatoid arthritis animal models.
Alternative names: KCNMA1 (KCa1.1), Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1 -
基因ID
- 16531
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NCBI登录号
- NM_002247
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UniProt
- Q08460-2
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途径
- Regulation of Hormone Metabolic Process, Sensory Perception of Sound
抗原
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