KCNK18 抗体 (1st Extracellular Loop)
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Quick Overview for KCNK18 抗体 (1st Extracellular Loop) (ABIN7043446)
抗原
See all KCNK18 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 1st Extracellular Loop, AA 62-75
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原理
- A Rabbit Polyclonal Antibody to KCNK18 Channel
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Unlikely to recognize human samples.
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预测反应
- Rat - 13,14 amino acid residues identical
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产品特性
- Anti-KCNK18 (TRESK) (extracellular) Antibody is directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (ABIN7043446, ABIN7045021 and ABIN7045022) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18
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亚型
- IgG
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anti-Potassium Channel Subfamily K Member 18 (KCNK18) (AA 168-197) antibody
KCNK18 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33126 unconjugated
anti-Potassium Channel Subfamily K Member 18 (KCNK18) (Center) antibodyKCNK18 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: 1:50
IHC: 1:100
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7236295)
Blocking Peptide: (ABIN7236295)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))
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别名
- KCNK18
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背景
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Synonyms: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1
Description: KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.
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基因ID
- 332396
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NCBI登录号
- NM_181840
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UniProt
- Q6VV64
抗原
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