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nectin-3 抗体 (Extracellular)

Name: nectin-3 抗体 (Extracellular)
SKU: ABIN7043441
Availability: OutOfStock

This anti-nectin-3 antibody is a 兔多克隆 antibody detecting nectin-3 in WB, IHC, FACS 和 IF. Suitable for 小鼠.

Western blot analysis of human U-87 MG glioblastoma cell line lysate: - 1. (nectin-3 抗体 (Extracellular))

Expression of Nectin-3 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Nectin-3/PVRL3 (extracellular) Antibody (ABIN7043441, ABIN7044696 and ABIN7044697), (1:100), followed by goat anti-rabbit-AlexaFluor-488. (nectin-3 抗体 (Extracellular))

IHC

Cell surface detection of Nectin-3 in live intact human Jurkat T-cell leukemia cells: (black line) Cells. (nectin-3 抗体 (Extracellular))

FACS

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) testis membranes: - 1,2. (nectin-3 抗体 (Extracellular))

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate: - 1,2. (nectin-3 抗体 (Extracellular))

5 images

产品编号 ABIN7043441

发货至: 中国

Quick Overview for nectin-3 抗体 (Extracellular) (ABIN7043441)

抗原

See all nectin-3 (NECTIN3) 抗体

nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This nectin-3 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Discover our top product NECTIN3 Primary Antibody

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AA 84-97, Extracellular

原理

A Rabbit Polyclonal Antibody to Nectin-3/PVRL3

特异性

Extracellular, N-terminus

交叉反应

人, 小鼠, 大鼠

预测反应

Human,rat - identical

产品特性

Anti-Nectin-3/PVRL3 (extracellular) Antibody (ABIN7043441, ABIN7044696 and ABIN7044697) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Nectin-3 from human, rat, and mouse samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: (C)ETITQISWEKIHGK, corresponding to amino acid residues 84-97 of mouse Nectin-3

亚型

IgG
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 282-404) antibody
NECTIN3 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6D5B2 unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 59-166) antibody
NECTIN3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D1 unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 90-293) antibody
NECTIN3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 310-549) antibody
NECTIN3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 1-366) antibody
NECTIN3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (Internal Region) antibody
NECTIN3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 171-377) antibody
NECTIN3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (Center) antibody
NECTIN3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 1-366) antibody
NECTIN3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-Nectin cell adhesion molecule 3 (NECTIN3) (Internal Region) antibody
NECTIN3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200

说明

Negative Control: (ABIN7235987)
Blocking Peptide: (ABIN7235987)

限制

仅限研究用
状态

Lyophilized

溶解方式

Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

别名

NECTIN3

背景

Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113,Nectins are Ca2+-independent immunoglobulin-like cell adhesion molecules that localize at adherens junctions between two neighboring cells. They play an important role in mediating asymmetric homotypic and heterotypic cell-cell adhesions. The nectin family contains four members, nectin-1-4, which are encoded by the PVRL1-4 genes, respectively. Nectin-3 is localized mostly in dendrites and partly in axons and is crucial for the specific interactions between axons and dendrites and proper formation of the synapse1,2. Its structure consists of an extracellular region with three immunoglobulin-like loops, one V type and two C2 types.Nectins binds afadin at its c-terminal region and connects to the actin cytoskeleton. This interaction plays a major role in the formation of various cell-cell junctions in cooperation with or independently of cadherins cell adhesion molecules1,2.Nectins also regulates numerous cellular functions, such as cell movement, proliferation, polarization, survival and differentiation by interacting with various proteins1.

Alternative names: Nectin-3/PVRL3, Nectin cell adhesion molecule 3, Poliovirus receptor-related 3, CD113

基因ID

58998

NCBI登录号

NM_015480

UniProt

Q9JLB9

途径

Cell-Cell Junction Organization